bbFISH-ing in the sonication fluid

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Using homogenization, sonication and thermo-sonication to inactivate fungi

Ultrasound (US), Thermo-sonication (TS) and High Pressure Homogenization (HPH) were studied as tools to inactivate the spores of Penicillium spp. and Mucor spp. inoculated in distilled water. For US, the power ranged from 40% to 100%, pulse from 2 to 10 s, and duration of the treatment from 2 to 10 min. TS was performed combining US (40-80% of power, for 8 min and pulse of 2 s) with a thermal t...

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Detection of bacteria with molecular methods in prosthetic joint infection: sonication fluid better than periprosthetic tissue

Background and purpose - The correct diagnosis of prosthetic joint infection (PJI) can be difficult because bacteria form a biofilm on the surface of the implant. The sensitivity of culture from sonication fluid is better than that from periprosthetic tissue, but no comparison studies using molecular methods on a large scale have been performed. We assessed whether periprosthetic tissue or soni...

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Molecular Diagnosis of Orthopedic-Device-Related Infection Directly from Sonication Fluid by Metagenomic Sequencing

Culture of multiple periprosthetic tissue samples is the current gold standard for microbiological diagnosis of prosthetic joint infections (PJI). Additional diagnostic information may be obtained through culture of sonication fluid from explants. However, current techniques can have relatively low sensitivity, with prior antimicrobial therapy and infection by fastidious organisms influencing r...

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Improved diagnosis of orthopedic implant-associated infection by inoculation of sonication fluid into blood culture bottles.

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ژورنال

عنوان ژورنال: Medicine

سال: 2019

ISSN: 0025-7974,1536-5964

DOI: 10.1097/md.0000000000016501